Task:1 Survival of Dissociated Neurons and Hippocampal Slice Cultures on Microelectrode Arrays

Survival of Dissociated Neurons and Hippocampal Slice Cultures on Microelectrode Arrays

Goal: To maintain cultures of both dissociated neurons and hippocampal slice cultures over a extended period of time.

Progress: Dissociated hippocampal neurons have been successfully grown on two microelectrode arrays, the Multichannel MEA 1060 and the Multiplate Multielectrode system from Dr. Gross from the University of North Texas. In addition, hippocampal slices were also cultured successfully on the Gross system. In addition to the long-term culture of hippocampal slices on membranes previously described, we are currently developing a version of the roller technique originally described by Gahwyler and colleagues to culture hippocampal slices.

Figure 1: Hippocampal slice maintained in culture for 2 weeks with a modified roller technique. Note the well-preserved organization with the dentate gyrus, fields CA3 and CA1.